Generator
Part:BBa_K802000:Design
Designed by: Carine Gimbert, Anne Haziza Group: iGEM12_Lyon-INSA (2012-09-20)
Lysostaphin generator for B. subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1316
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Used a strong RBS in order to produce high levels of expression of Lysostaphin.
Source
This is a composite part including the constitutive B. subtilis Pveg promotor and lysostaphin coding sequence.